We recommend using Random Primer Mix for reverse transcription of the following RNA templates:
RNA without poly(A) tail such as ribosomal RNAs.
RNA with strong secondary structures.
Partially degraded RNA samples.
Target regions at 5´ end of a long messenger RNA transcript.
Figure 1:Various amounts of human spleen total RNA (2 μg to 20 pg) were reverse transcribed using ProtoScript First Strand cDNA Synthesis Master Mix Kit (NEB #E6300 ) in the presence of 6 μM Random Primer Mix (panel A) or 3.5 μM random hexamers (panel B). About 1/10th of the cDNA products were then analyzed in duplicate qPCR reactions using a pair of primers specific for the beta actin gene with DyNAmo™ FLASH SYBR® Green qPCR Master Mix on a Bio-Rad IQ5® system.
This product is related to the following categories:
Reverse Transcriptases & RT-PCR Products
This product can be used in the following applications: