TriDye™ 1 kb DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNAincludes fragments ranging from 0.5-10.0kilobases. The 3.0 kb fragment has increased intensity to serve as a reference band.
Recommended gel percentage range: 0.8-1.2%
Optimum separation on 1%
TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.1 kb DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel. Mass values are for 0.5 μg/lane.
This product is related to the following categories:
DNA Markers & Ladders Products
This product can be used in the following applications: