Description:
OneTaq?HotStart2XMasterMixwithStandardBufferisanoptimizedblendofTaqandDeepVentR?DNAPolymerasescombinedwithanaptamer-basedinhibitor.ThisenzymeblendisideallysuitedtoroutinePCRapplicationsfromavarietyoftemplates,includingpureDNAsolutions,bacterialcoloniesandCDNAproducts.The3′→5′exonucleaseactivityofDeepVentDNAPolymeraseincreasesthefidelityandrobustamplificationofTaqDNAPolymerase(1).Thehotstartnatureoftheenzymeoffersconveniencewithdecreasedinterferencefromprimerdimersandsecondaryproducts.TheconvenientmastermixformulationcontainsdNTPs,MgCl2andotherbuffercomponentsandstABIlizerslistedbelow,requiringonlytheadditionofprimersandDNAtemplateforrobustamplification
AmplificationofaselectionofsequenceswithvaryingGCcontentfromhumanandC.elegansgenomicDNAusingOneTaqHotStart2XMasterMixwithStandardBuffer.Thepresenceofabsenceofanextendedroomtemperatureincubationdoesnotaffectperformance.Ampliconsizesareindicatednexttogel,andGCcontentisindicatedbelowgel.MarkerMisthe1kbDNALadder(NEB#N3232).
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AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticusYT-1andan?E.colistrainthatcarriestheDeepVentRDNAPolymerasegenefromPyrococcusspeciesGB-D.Notes:
ProductspecificationsforindividualcomponentsintheOneTaqHotStart2XMasterMixwithStandardBufferareavailableseparately.OneTaqHotStart2XMasterMixwithStandardBufferisstableforfifteenfreeze-thawcycleswhenstoredat-20°C.OneTaqHotStart2XMasterMixwithStandardBufferisalsostableforonemonthat4°C,soforfrequentuse,analiquotmaybekeptat4°C.