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OneTaq?RT-PCRKitcombinestwopowerfulmixes,M-MuLVEnzymeMixandOneTaqHotStart2XMasterMixwithStandardBufferfor2-stepRT-PCRapplications.ThetwomixesrequireminimalhandlingduringreactionsetupandyetofferconsistentandrobustRT-PCRreactions.ThefirststrandCDNAsynthesisisachievedbyusingtwooptimizedmixes,M-MuLVEnzymeMixandM-MuLVReactionMix.M-MuLVEnzymeMixcombinesM-MuLVReverseTranscriptaseandmurineRNaseInhibitorwhileM-MuLVReactionMixcontainsdNTPsandanoptimizedbuffer.Thekitalsocontainstwooptimizedprimersforreversetranscriptionandnuclease-freewater.Ananchoredoligo-dTprimer[d(T)23VN]forcestheprimertoannealtothebeginningofthepolyAtail.TheoptimizedRandomPrimerMixprovidesrandomandconsistentprimingsitescoveringtheentireRNAtemplatesincludingbothmRNAsandnon-polyadenylatedRNAs.TheamplificationstepfeaturesaOneTaqHotStartDNAPolymeraseinamastermixformat.OneTaqHotStartDNAPolymeraseoffershigherfidelitythanTaqandbetteramplification.RT-PCRproductupto6kbcanbegenerated(Figure1).
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