Rabbit antisera. Liquid. Contains no preservative.
Storage and Shipping Information
Storage Conditions
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Applications
Application
Anti-Galactocerebroside Antibody detects level of Galactocerebroside & has been published & validated for use in ELISA, IC, IH.
Key Applications
ELISA
Immunocytochemistry
Immunohistochemistry
Applications Not Recommended
Immunohistochemistry (Paraffin)
Application Notes
Immunohistochemistry: suggested starting dilution 1:10-1:50. Not suggested for use on paraffin embedded tissue sections.
Immunocytochemistry: suggested starting dilution 1:10-1:50. For staining of living cells it is recommended that the antisera be heat inactived prior to using to remove complement. Staining of living cells prior to fixation will show a typical "patching" appearance. Cells fixed prior to staining will have a more solid, "smooth" appearance.
The diluent of choice is DMEM but other buffers may be used.
The recommended fixative is 4% paraformaldehyde.
ELISA: suggested starting dilution 1:50-1:100.
Optimal working dilutions must be determined by end user.
Biological Information
Immunogen
Galactocerebroside purified from bovine brain.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
Recognizes Oligodendrocytes in mouse brain culture cells (live). AB142 has also shown staining on fixed mouse brain tissue. Other possible applications are staining of rat and human living brain cells / tissue.